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KMID : 1101320070390030147
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Korean Journal of Clinical Laboratory Science
2007 Volume.39 No. 3 p.147 ~ p.150
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Genotyping of HLA-B by Polymerase Chain Reaction-Sequence Specific Primer
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Jang Soon-Mo
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Abstract
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Most expressed HLA (human leukocyte antigen) loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this study, the HLA-B genotypes were determined in twenty students unrelated koreans using the PCR-SSP (polymerase chain reaction-sequence specific primer) technique. Several specific primer pairs in assigning the HLA-B gene were used (B*4001/4007, B*4901/5001/4501, B*3701, B*5801). The results of PCR-SSP, the HLA-B3701 primer was detected one (5%), the HLA-B*5801 were detected four (20%), the HLA-B*4001/4007 were detected nineteen (95%) and the HLA-B*4901/5001/ 4501 were detected twenty. This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-B genotypes. Moreover, these results genotype frequency of the HLA-B gene could be useful for database study before being applied to individual identification and transplantation immunity.
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KEYWORD
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HLA, HLA-B*4001/4007, HLA-B*3701, HLA-B*4901/5001/4501, HLA-B*5801, PCR-SSP
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